Ribosite Mycoplasma synoviae Real-time PCR Detection kit

Ribosite® Mycoplasma synoviae kits are intended for the specific detection of bacterial DNA of Mycoplasma synoviae (MS). The kits are suitable for the analysis of samples from birds such as swab samples (e.g. nasal, choanal, tracheal, cloacal, from air sacs, lungs or conjunctiva), tissues and organs (e.g. trachea, lung) and pure or mixed colony material / isolates derived from cultural processes of the aforementioned samples. BACKGROUND RIBOSITEBIOTECHNOLOGY.COM Ribosite Mycoplasma synoviae Real-time PCR Detection kit ® Ribosite® Mycoplasma synoviae kit utilizes a duplex Real-Time PCR to provide a qualitative testing method. The PCR reaction amplifies target genes for both Mycoplasma synoviae and an exogenous control, the Internal Amplification Control (IAC), in a single reaction setting using specific primer pairs. During the PCR reaction, fluorescently labeled probes detect the amplified target gene fragments in real-time, with the probe for Mycoplasma synoviae labeled with FAM and the probe for the exogenous control labeled with HEX. The emitted fluorescence is separately measured by the Real-Time PCR thermal cycler. Each sample is individually analyzed in one reaction vessel, with a Negative Control and Positive Control run alongside to aid in evaluating the Mycoplasma synoviae-specific status of the sample. With this method, results can be obtained in just a few hours after the sample is received.